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101.
The effects of progesterone, 20α-hydroxy-pregn-4-en-3-one, (20α-OH-P) 17 α hydroxyprogesterone (17α-OH-P) and 5α-pregnan-3, 20-dione (5α-P) on endometrium of ovariectomized rabbits have been examined. Progesterone, at 5 mg/kg/day and at 1.7 mg/kg/day increased the number of mitotic figures observed in luminal and glandular epithelium after 5 days of treatment and induced considerable arborization. None of the other compounds induced significant arborization and only 10 mg/kg/day of 20α-OH-P increased the mitotic index in luminal and glandular epithelium. Administrations of 20α-OH-P, 17α-OH-P or 5α-P resulted in the appearance of very large, pale cells in the luminal epithelium which were not present in progesterone treated rabbits. Cytochemical techniques revealed no specific staining in these cells for polysaccharides (PAS, Alcian blue), lipid (Oil Red O) or for blastokinin, a progesterone-inducible, uterine protein, examined by the fluoroscein isothiocyanate labeled antibody technique. The possibility of an apparent dissociation between histodifferentiation and cytodifferentiation is discussed. 相似文献
102.
Mycobacterium sp. NRRL B-3683 converted tall oil sterols to C19 steroids as efficiently as it converted soybean sterols. 相似文献
103.
Megan K. Meany William R. Conner Sophia V. Richter Jessica A. Bailey Michael Turelli Brandon S. Cooper 《Evolution; international journal of organic evolution》2019,73(6):1278-1295
Maternally transmitted Wolbachia bacteria infect about half of all insect species. Many Wolbachia cause cytoplasmic incompatibility (CI) and reduced egg hatch when uninfected females mate with infected males. Although CI produces a frequency‐dependent fitness advantage that leads to high equilibrium Wolbachia frequencies, it does not aid Wolbachia spread from low frequencies. Indeed, the fitness advantages that produce initial Wolbachia spread and maintain non‐CI Wolbachia remain elusive. wMau Wolbachia infecting Drosophila mauritiana do not cause CI, despite being very similar to CI‐causing wNo from Drosophila simulans (0.068% sequence divergence over 682,494 bp), suggesting recent CI loss. Using draft wMau genomes, we identify a deletion in a CI‐associated gene, consistent with theory predicting that selection within host lineages does not act to increase or maintain CI. In the laboratory, wMau shows near‐perfect maternal transmission; but we find no significant effect on host fecundity, in contrast to published data. Intermediate wMau frequencies on the island of Mauritius are consistent with a balance between unidentified small, positive fitness effects and imperfect maternal transmission. Our phylogenomic analyses suggest that group‐B Wolbachia, including wMau and wPip, diverged from group‐A Wolbachia, such as wMel and wRi, 6–46 million years ago, more recently than previously estimated. 相似文献
104.
Kyle A. Hollister Elizabeth S. Conner Xinxing Zhang Mark Spell Gary M. Bernard Pratik Patel Ana Carolina G.V. de Carvalho Rebecca A. Butcher Justin R. Ragains 《Bioorganic & medicinal chemistry》2013,21(18):5754-5769
The nematode Caenorhabditis elegans secretes ascarosides, structurally diverse derivatives of the 3,6-dideoxysugar ascarylose, and uses them in chemical communication. At high population densities, specific ascarosides, which are together known as the dauer pheromone, trigger entry into the stress-resistant dauer larval stage. In order to study the structure–activity relationships for the ascarosides, we synthesized a panel of ascarosides and tested them for dauer-inducing activity. This panel includes a number of natural ascarosides that were detected in crude pheromone extract, but as yet have no assigned function, as well as many unnatural ascaroside derivatives. Most of these ascarosides, some of which have significant structural similarity to the natural dauer pheromone components, have very little dauer-inducing activity. Our results provide a primer to ascaroside structure–activity relationships and suggest that slight modifications to ascaroside structure dramatically influence binding to the relevant G protein-coupled receptors that control dauer formation. 相似文献
105.
Gregory B. Noe Ken W. Krauss B. Graeme Lockaby William H. Conner Cliff R. Hupp 《Biogeochemistry》2013,114(1-3):225-244
Tidal freshwater wetlands are sensitive to sea level rise and increased salinity, although little information is known about the impact of salinification on nutrient biogeochemistry in tidal freshwater forested wetlands. We quantified soil nitrogen (N) and phosphorus (P) mineralization using seasonal in situ incubations of modified resin cores along spatial gradients of chronic salinification (from continuously freshwater tidal forest to salt impacted tidal forest to oligohaline marsh) and in hummocks and hollows of the continuously freshwater tidal forest along the blackwater Waccamaw River and alluvial Savannah River. Salinification increased rates of net N and P mineralization fluxes and turnover in tidal freshwater forested wetland soils, most likely through tree stress and senescence (for N) and conversion to oligohaline marsh (for P). Stimulation of N and P mineralization by chronic salinification was apparently unrelated to inputs of sulfate (for N and P) or direct effects of increased soil conductivity (for N). In addition, the tidal wetland soils of the alluvial river mineralized more P relative to N than the blackwater river. Finally, hummocks had much greater nitrification fluxes than hollows at the continuously freshwater tidal forested wetland sites. These findings add to knowledge of the responses of tidal freshwater ecosystems to sea level rise and salinification that is necessary to predict the consequences of state changes in coastal ecosystem structure and function due to global change, including potential impacts on estuarine eutrophication. 相似文献
106.
M. O’Callaghan E. M. Gerard N. W. Waipara S. D. Young T. R. Glare P. J. Barrell A. J. Conner 《Plant and Soil》2005,266(1-2):47-56
Antimicrobial peptide magainin II, isolated from the skin of the African clawed toad, has shown activity in vitro against a range of micro-organisms. Transgenic potato lines expressing a synthetic magainin gene show improved resistance
to the bacterial plant pathogen, Erwinia carotovora. Culturable bacterial and fungal communities associated with magainin-producing potato plants were compared with those communities
from the non-transgenic parental control and with another potato cultivar. Total numbers of aerobic bacteria recovered from
the leaves of the magainin-producing line, its non-transgenic parent line and an unrelated cultivar did not differ significantly.
There were no detectable differences in the numbers of Gram-positive and Gram-negative bacteria, pseudomonad populations or
fungi recovered from foliage from the three plant lines. Bacterial populations recovered from the roots of a magainin-expressing
plant line did not differ significantly from populations recovered from the unmodified parental line. Tubers from the magainin-expressing
transgenic potatoes, however, had significantly lower total numbers of bacteria than tubers produced by unmodified plants.
In vitro testing of rhizosphere isolates against magainin analogues found that bacterial isolates varied in their susceptibility to
the peptides. There were no significant differences in the total numbers of fungi and yeasts recovered from the various plant
lines, with one exception: higher numbers of fungi were recovered from roots of magainin-expressing plants than the unmodified
control plants. 相似文献
107.
108.
Jeffrey Conner 《Evolution; international journal of organic evolution》1988,42(4):736-749
Selection on three phenotypic traits was estimated in a natural population of a fungus beetle, Bolitotherus cornutus. Lifetime fitness of a group of males in this population was estimated, and partitioned into five components: lifespan, attendance at the mating area, number of females courted, number of copulations attempted, and number of females inseminated. Three phenotypic characters were measured—elytral length, horn length, and weight; there were strong positive correlations among the three characters. Selection was estimated by regressing each component of fitness on the phenotypic traits. Of the three traits, only horn length was under significant direct selection. This selection was for longer horns and was due mainly to differences in lifespan and access to females. The positive selection on horn length combined with the positive correlations between horn length and the other two characters resulted in positive total selection on all three characters. 相似文献
109.
Conner JK Karoly K Stewart C Koelling VA Sahli HF Shaw FH 《The American naturalist》2011,178(4):429-441
Genetic correlations are the most commonly studied of all potential constraints on adaptive evolution. We present a comprehensive test of constraints caused by genetic correlation, comparing empirical results to predictions from theory. The additive genetic correlation between the filament and the corolla tube in wild radish flowers is very high in magnitude, is estimated with good precision (0.85 ± 0.06), and is caused by pleiotropy. Thus, evolutionary changes in the relative lengths of these two traits should be constrained. Still, artificial selection produced rapid evolution of these traits in opposite directions, so that in one replicate relative to controls, the difference between them increased by six standard deviations in only nine generations. This would result in a 54% increase in relative fitness on the basis of a previous estimate of natural selection in this population, and it would produce the phenotypes found in the most extreme species in the family Brassicaceae in less than 100 generations. These responses were within theoretical expectations and were much slower than if the genetic correlation was zero; thus, there was evidence for constraint. These results, coupled with comparable results from other species, show that evolution can be rapid despite the constraints caused by genetic correlations. 相似文献
110.
The plasmodiophorids are a phylogenetically distinct group of parasitic protists that infect plants and stramenopiles, causing several important agricultural diseases. Because of the obligate intracellular part of their lifecycle, none of the plasmodiophorids has been axenically cultured. Further, the molecular biology of the plasmodiophorids is poorly understood because pure cultures are not available from any species. We report on an in-vitro dual culture system of the plasmodiophorids Plasmodiophora brassicae and Spongospora subterranea with their respective plant hosts, Brassica rapa and Solanum tuberosum. We show that these plasmodiophorids are capable of initiating and maintaining stable, long-term plant cell callus cultures in the absence of exogenous plant growth regulators. We show that callus cultures harbouring S. subterranea provide an excellent starting material for gene discovery from this organism by constructing a pilot-scale DNA library. Bioinformatic analysis of the sequences established that almost all of the DNA clones from this library were from S. subterranea rather than the plant host. The Spongospora genome was found to be rich in retrotransposable elements, and Spongospora protein-coding genes were shown to contain introns. The sequence of a near full-length non-LTR retrotransposon was obtained, the first transposable element reported from a cercozoan protist. 相似文献